Protein Characterization Service |
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Protein Characterization
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Proteome Factory offers characterization of proteins and antibodies by most modern mass spectrometric techniques in combination with HPLC and N-terminal peptide sequencing (Edman degradation). Proteins are digested by different endoproteases and/or chemical cleaving methods to generate overlapping peptides of the protein. Following HPLC separation of the different digests the peptides are sequenced by Edman degradation, analyzed by MALDI-MS and MSMS techniques. To ensure highest sensitivity and reliabililty Proteome Factory uses nanoLC-ESI-MSMS and/or MALDI-TOF-TOF-MS which allow de novo peptide sequencing by MSMS analysis in combination with de novo sequencing software SEQUiT!. By combination of the peptide sequence data the protein's sequence is determined. |
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