MeCAT Proteomik Kits |
||||||||||||||||
MeCAT Duplex Kit für die Proteomik
|
||||||||||||||||
Das MeCAT Duplex Kit wurde für die differentielle Markierung von Proteinen und Proteingemischen entwickelt, um die Proteinprofile (Proteome) von zwei Proben quantitativ zu vergleichen. Mittels ICP-MS ist eine absolute Quantifizierung von Proteinen möglich. |
||||||||||||||||
|
||||||||||||||||
MeCAT Workflow
|
||||||||||||||||
|
MeCAT - Metal Coded Tagging Technolgy for (absolute) protein quantification. Presentation at DGMS Workshop Quantitative Proteomics, Bremen 2007 (pdf document) |
|||||||||||||||
Example MeCAT Workflow Ultra sensitive absolute and relative MeCAT protein quantification is performed by ICP-MS (inductively coupled plasma mass spectrometry) on protein level down to low attomol range. Proteins can be identified and relatively quantified on peptide level by MALDI-MS an ESI-MSMS. Metal standard solutions are used for high accuracy external protein independent calibration of ICP-MS. |
||||||||||||||||
(C) 2006 - Proteome Factory AG - All rights reserved |