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SEQUiT! in brief

SEQUiT 4.1 - de novo peptide sequencing software is ...

... precise

in computation of complete peptide sequences
in successful sequence computing from tandem mass spectrometry data using even incomplete b- or y-fragment series
in accurate sequencing of tryptic and nontryptic (e.g. MHC peptides) peptides
in sequencing of chemically or post-translationally modified peptides
in different scoring systems for ESI- and MALDI-MS techniques (already successfully tested with data provided by MALDI-QTOF, MALDI-TOF (Post Source Decay), MALDI-TOF/ TOF (Laser Induced Dissociation, Collision Induced Dissociation), ESI-TRAP, ESI-QTOF and ESI-FTICR)

in extending individual amino acid list
in extendending own list of modifications
in using BLAST search engine in combination with different databases and taxonomy restrictions by GI (GenBank identifier) lists

SEQUiT! supports common MS/MS data formats (e.g. *.sqi, *.mgf, *.dta, *.pkl)
You can process batch data: de novo sequencing with subsequent database searches
SEQUiT! generates report files with batch analysis results

SEQUiT 4.1 allows intuitionally and uncomplicated usage.

SEQUiT! software
Demine R., Walden P. Sequit: software for de novo peptide sequencing by matrix-assisted laser desorption/ionization post-source decay mass spectrometry. Rapid Commun Mass Spectrom. 2004; 18: 907-913.

Used in
Demine R., Walden P. Testing the role of gp96 as peptide chaperone in antigen processing. J Biol Chem. 2005; 280: 17573-17578.

Hardware/software configuration
Minimal: Pentium 4 CPU 1.70 MHz, 512 MB RAM, Windows 2000 or Windows XP, IE 6.0
Optimal: Pentium 4 CPU 3.40 MHz, 2 GB RAM, Windows 2000 or Windows XP, IE 6.0

Pentium is a registered trademark of Intel Corporation.
Windows 2000 and Windows XP are registered trademarks of Microsoft Corporation

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